Adiponectin Rabbit Polyclonal Antibody

SKU: orb1294234

Description

Rabbit polyclonal antibody to Adiponectin

Research Area

Metabolism Research

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500 – 1:1000, Immunofluorescence 1:200 – 1:300, Immunohistochemistry (Paraffin) 1:100 – 1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide/adiponectin, C1Q and collagen domain containing
Target	Adiponectin
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Anti-Adiponectin antibody at 1/1000 dilution, Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294234 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Adiponectin with orb1294234 at 1/100.

Quick Database Links

Gene Symbol

Adiponectin

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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