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ALPPL2 Rabbit Polyclonal Antibody
SKU: orb312168
Featured
Description
Images & Validation
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| Tested Applications | FC, IF, IHC-Fr, IHC-P |
|---|---|
| Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
| Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ALPPL2 (301-400/532aa) |
| Target | ALPG |
| Molecular Weight | 53 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Akp5; Alkaline phosphatase; Alkaline phosphatase Nagao isozyme; Alkaline phosphatase placental like; ALP 1; ALP-1; ALP1; ALPG; ALPPL 2; ALPPL; C77216; D1Ertd816e; GCAP; Germ cell alkaline phosphatase; Nagao isozyme; Placental alkaline phosphatase-like; Placental like alkaline phosphatase; placental-like; PLAP like; PLAP-like; PPBN_HUMAN; Testicular and thymus alkaline phosphatase.
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Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C). Primary Antibody (green line): Rabbit Anti-ALPPL2 antibody (orb312168), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ALPPL2 Polyclonal Antibody, Unconjugated (orb312168) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
ALPG
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ALPPL2 Rabbit Polyclonal Antibody (orb312168)
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