Anti-4-1BBL [AT113-2]

SKU: orb758809

Description

Rabbit monoclonal antibody to 4-1BBL

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	Blocking, FC, WB
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG
Clone No.	AT113-2
Immunogen	This antibody was raised by immunising LOU rats with soluble recombinant 4-1BBL:Fc fusion protein.
Target	4-1BBL
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD137L; TNFSF9; 4-1BB ligand; 41BBL; CDw137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; tumor necrosis factor (ligand) superfamily member 9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand 5A; Tumor necrosis factor ligand superfamily member 9.

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Flow cytometry using the anti- 4-1BBL antibody AT113-2. RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of AT113-2 (orb758809, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-4-1BBL antibody AT113-2. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of AT113-2 (orb758809) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758809, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

4-1BBL

UniProt

P41274

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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