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Description
Research Area
Immunology & Inflammation; Cell Biology
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, FC, IHC, WB |
|---|---|
| Dilution Range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | BFO71 |
| Target | CD27 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD27, TNFRSF7, S152, T14, Tp55
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Expi 293 cell line transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1 μg/ml (clone: DM59) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Flow cytometry data of serially titrated Rabbit anti-CD27 monoclonal antibody (clone: DM59) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit anti-CD27 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Flow cytometry analysis of antigen binding of rabbit anti-human CD27 mAb (orb757382). (A) orb757382 does not bind to 293T cells that do not express CD27. (B) A clear peak shift of orb757382 was seen compared to the control when incubated with CD27-expressing Raji cells, indicating strong binding of orb757382 to CD27. Antibodies were incubated at 2 μg/mL.
Anti-CD27 antibody (SKU# orb757382) at 1/1000 dilution.Lane: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 29 kDa.Observed band size: 55 kDa
A. orb757382 at 10 μg/ml staining CD27 in Raji cells by IHC (SKU# orb757382); B. orb757382 at 10 μg/ml staining CD27 in human tonsil tissue by IHC (SKU# orb757382).
Quick Database Links
Gene Symbol
CD27
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD27 Antibody (orb757382)
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