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CD276 Antibody
SKU: orb1473751
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1/1000 - 1/2000), IH (1/50 - 1/200) |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Recombinant fusion protein of human CD276. The exact sequence is proprietary. |
| Purification | This antibody is purified through a protein G column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−B7H3; CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD276
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Western blot analysis of CD276 expression in 293 (A), A431 (B), U20S (C), LNCap (D), Hela (E), MCF7 (F) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 100 kD)
Immunohistochemical analysis of CD276 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
CD276 Antibody (orb1473751)
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