CD3 Antibody-FITC labled

SKU: orb1293915

Description

The CD3 Antibody-FITC labled is suitable for FC, IF. It is a Monoclonal, FITC conjugated antibody which raised against γδTCR-positive T-T hybridoma D1. Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF
Dilution range	10 μl / assay
Reactivity	Mouse
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rat
Clonality	Monoclonal
Clone No.	17A2
Immunogen	γδTCR-positive T-T hybridoma D1
Purification	The antibody was purified by affinity chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Rat IgG2b kappa. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Disclaimer	For research use only

Alternative Names

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T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; T3G; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD3g

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Quick Database Links

UniProt

P22646, P11942

NCBI Gene ID

12501, 12502

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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