CD3 Mouse Monoclonal Antibody (PerCP/Cy5.5)

SKU: orb1293490

Description

The CD3 Antibody-PerCP/Cy5.5 labled is suitable for FC, IF. It is a Monoclonal, PerCP/Cy5.5 conjugated antibody which raised against Native purified human CD3.. Purification: The antibody was purified by affinity chromatography.

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	FC, IF
Dilution Range	10 μl / assay
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a
Clone No.	OKT3
Immunogen	Native purified human CD3.
Target	CD3D; CD3E; CD3G; CD247
Purification	The antibody was purified by affinity chromatography.
Conjugation	PerCP/Cy5.5

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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T3D; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD antigen CD3d; T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD antigen CD3e; T3G; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD antigen CD3g; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247

Quality Guarantee

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Quick Database Links

Gene Symbol

CD3D; CD3E; CD3G; CD247

UniProt

P07766, P04234, P09693, P20963

NCBI Gene ID

916, 917, 919, 915

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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