Anti-CD3 epsilon [12F6]

SKU: orb411584

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Description

Mouse monoclonal antibody to CD3 epsilon

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Tested Applications	FC
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG
Clone No.	12F6
Immunogen	Human CD3
Target	CD3 epsilon
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Leu4/T3; ET-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e

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Flow-cytometry using anti-CD3 antibody 12F6 Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of 12F6 (orb411584, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

CD3 epsilon

UniProt

P07766

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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Li, Bohua et al. Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation functions Immunology, 116, 487-498 (2005)

PubMed: 16313362