CD3 epsilon (activation epitope) Antibody

SKU: orb44679

Description

Mouse monoclonal antibody to CD3

Research Area

Epigenetics, Immunology, Stem Cells

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, IHC-Fr, IP, WB
Reactivity	Human, Mouse
Application Notes	Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml; positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/ml). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. Read more...

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	APA1/1
Immunogen	Purified human CD3 proteins isolated from thymus
Target	CD3 epsilon (activation epitope)
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD3 epsilon chain, T3E, IMD18

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CD3 epsilon (activation epitope) Antibody

Separation of Jurkat cells stained anti-human CD3 activation epitope (APA1/1) purified antibody (concentration in sample 5 µg/ml, GAM FITC, red-filled) from Jurkat cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).