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CD3 epsilon Rabbit Monoclonal Antibody
SKU: orb548078
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IF, IHC, IP |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | COB-3 |
| Immunogen | A synthesized peptide derived from human CD3 epsilon |
| Molecular Weight | 250 kDa |
| Purification | Affinity-chromatography |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Concentration | 0.5mg/ml |
| Disclaimer | For research use only |
Alternative Names
−T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3E; T3E
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Immunohistochemical analysis of paraffin embedded mouse spleen labelling CD3 epsilon (orb548078) CD3E was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD3E Antibody (orb548078) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.
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Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
CD3 epsilon Rabbit Monoclonal Antibody (orb548078)
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