Anti-CD3 epsilon [UCHT1]

SKU: orb420102

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Description

Rabbit monoclonal antibody to CD3 epsilon

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	FC, IHC
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG
Clone No.	UCHT1
Immunogen	Human infant thymocytes.
Target	CD3 epsilon
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD3e; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3-ε;

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Flow-cytometric analysis of the dose-dependency (D, F, H) of anti-hCD3e antibody binding to live human PBMCs (B). The gating strategy is shown for control puposes (A, B, C, E, G).

Western Blot analysis (3 µg/ml) staining of Jurkat (A) and Molt4 (B) lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CD3 epsilon

UniProt

P07766

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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Flow Cytometry

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Find More Protocols

Arnett, Kelly L. et al. Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment Proc Natl Acad Sci U S A, 101, 16268-16273 (2004)

PubMed: 15534202