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Anti-CD3
SKU: orb1132738
Description
Images & Validation
−
| Tested Applications | FC, IP |
|---|---|
| Dilution range | - Flow Cytometry 1:500 (Positive control: Peripheral Blood Lymphocytes, JURKAT human leukemia T cell line) - Immunoprecipitation: The antibody immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4. - Functional Application: The antibody has strong mitogenic effect on peripheral T lymphocytes; it reacts strongly with gamma delta T lymphocytes. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Human thymocytes and T lymphocytes |
| Purity | Affinity purified |
| Purification | Affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, pH 7.4 with 0.05% sodium azide. |
| Concentration | 1ug/ul |
| Disclaimer | For research use only |
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
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Protocol Information
FC
Flow Cytometry
IP
Immunoprecipitation
Anti-CD3 (orb1132738)
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