Anti-CD31 [BAG-85D10]

SKU: orb758908

Description

Mouse monoclonal antibody to CD31

Images & Validation

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Tested Applications	IHC
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	BAG-85D10
Immunogen	This antibody was raised by immunising mice with human peripheral blood lymphocytes from a patient with high numbers of gamma-delta T cells.
Target	CD31
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; JC/70A; BAG 85D10

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Flow cytometry using the Anti-CD31 antibody BAG-85D10. Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of BAG-85D10 (orb758909, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed K562 cells with anti-CD31 antibody BAG-85D10. Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BAG-85D10 (orb758909) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758909, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD31

UniProt

P16284

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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