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Anti-CD3e [33H1]
SKU: orb1821579
Description
Research Area
Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | 33H1 |
| Immunogen | The original antibody was generated by immunizing BALB/c mice with recombinant human CD3 antigen (amino acids 23-126). |
| Target | CD3e |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3-ε; CGMCC NO 21980; SP7
Similar Products
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![Anti-CD3e [33H1]](/images/pub/media/catalog/product/NewWebsite/35/orb1821580_1.png)
![Anti-CD3e [33H1]](/images/pub/media/catalog/product/NewWebsite/35/orb1821580_2.png)
Quality Guarantee
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![Anti-CD3e [33H1]](/images/pub/media/catalog/product/NewWebsite/35/orb1821579_1.png)
Flow cytometry using anti-CD3e antibody 33H1. Jurkat cells were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 33H1 (orb1821579, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.
![Anti-CD3e [33H1]](/images/pub/media/catalog/product/NewWebsite/35/orb1821579_2.png)
Immunofluorescence staining of Jurkat cells with anti-CD3e antibody 33H1. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 33H1 (orb1821579) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821579, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
CD3e
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Anti-CD3e [33H1] (orb1821579)
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