Anti-CD3e [898H2-6-15]

SKU: orb1152492

Description

Mouse monoclonal antibody to CD3e

Research Area

Immunology & Inflammation

Images & Validation

−

Item 1 of 3

Tested Applications	FC, IHC
Reactivity	Porcine

Key Properties

−

Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	898H2-6-15
Immunogen	Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.
Target	CD3e
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Leu4/T3; Cluster of Differentiation 3 epsilon chain; T cell glycoprotein CD3 epsilon chain

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow cytometry using the Anti-CD3e antibody 898H2-6-15. Paraformaldehyde fixed pig peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 898H2-6-15 (orb1152491, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of pig peripheral blood monocytes with anti-CD3e 898H2-6-15. Immunofluorescence analysis of paraformaldehyde fixed pig peripheral blood monocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 898H2-6-15 (orb1152491) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152491, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using Anti-CD3e antibody 898H2-6-15. Pig spleen(A) (1 µg/ml), pig thymus(B) (0.1 µg/ml) and lymph node(C) (0.1 µg/ml) tissue lysate (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 898H2-6-15 (orb1152491) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

CD3e

UniProt

Q7YRN2

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC

Immunohistochemistry

View Protocol

Flow Cytometry

View Protocol

Find More Protocols