CD86 Rat Monoclonal Antibody (FITC)

SKU: orb1293882

Description

The CD86 Antibody-FITC labled is suitable for FC, IF. It is a Monoclonal, FITC conjugated antibody which raised against LPS-activated CBA/Ca mouse splenic B cells. Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF
Dilution Range	10 μl / assay
Reactivity	Mouse
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG2a kappa
Clone No.	GL-1
Immunogen	LPS-activated CBA/Ca mouse splenic B cells
Target	CD86
Purification	The antibody was purified by affinity chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86

Quality Guarantee

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Quick Database Links

Gene Symbol

CD86

UniProt

P42082

NCBI Gene ID

12524

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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