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Cytokeratin 8 Antibody
SKU: orb43712
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, IHC-P, IP, WB |
|---|---|
| Reactivity | Bovine, Human, Porcine, Rabbit, Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | C-43 |
| Immunogen | Cytoskeleton preparation from HeLa human cervix carcinoma cell line. |
| Target | Cytokeratin 8 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−K8, CK8, CYK8, KRT8, K2C8, CARD2
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Western blotting detection of cytokeratin 8 in HeLa cell lysate (reducing conditions) by mouse monoclonal antibody C-43.
Immunocytochemistry staining of cytokeratin 8 in Hep-2 cells using mouse monoclonal C-43 (orb43712, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).
Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 8(C-43).
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
Cytokeratin 8 Antibody (orb43712)
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