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Anti-EDAR [EDAR12]
SKU: orb613920
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Canine, Gallus, Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | EDAR12 |
| Immunogen | EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA. |
| Target | EDAR |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ectodysplasin A receptor; Tumor necrosis factor receptor superfamily member EDAR; Anhidrotic ectodysplasin receptor 1; Downless; Ectodermal dysplasia receptor; Ectodysplasin-A receptor
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Anti-EDAR [EDAR12] [orb613921]
ELISA, WB
Canine, Gallus, Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
0.2 mg
![Anti-EDAR [EDAR12]](/images/pub/media/catalog/product/NewWebsite/35/orb613921_1.png)
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![Anti-EDAR [EDAR12]](/images/pub/media/catalog/product/NewWebsite/35/orb613920_1.png)
Immunofluorescence staining of fixed A549 cells with anti-EDAR antibody EDAR12. Immunofluorescence analysis of paraformaldehyde fixed A549 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of EDAR12 (orb613920) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing weak membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613920, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
EDAR
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Anti-EDAR [EDAR12] (orb613920)
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