EGFR Antibody [KO/KD Validated]

SKU: orb665717

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Description

The EGFR Antibody [KO/KD Validated] is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGFR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, Developmental Biology, Epigenetics, Infectious Diseases

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	WB: 1-500-1000, IHC-P: 1-50-200
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGFR. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of EGFR expression in HepG2 (A), mouse embryo (B) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)

Immunohistochemical analysis of EGFR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

Q01279, P00533

NCBI Gene ID

13649, 1956

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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