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EGFRvIII antibody
Description
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB: 2 μg/ml, IHC-P: 1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG2a |
| Immunogen | KLH conjugated synthetic peptide derived from human EGFRvIII. Please contact us for the exact immunogen sequence. The peptide is available as orb374827. |
| Target | EGFRvIII |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
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100 μg, 1 mgRecombinant EGFRvIII (Epidermal Growth Factor Receptor, Variant III) Antibody [orb751341]
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100 μg (without BSA and Azide), 100 μg, 20 μgERBB1 / EGFR / HER1 Reference Antibody [orb1817590]
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100 μg

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WB analysis of human breast cancer tissue using EGFRvIII (dilution of primary antibody - 2 ug/ml)

Immunohistochemical staining of paraffin embedded human endometrial cancer tissue using anti-EGFRvIII (primary antibody at 1:200)

IHC-P staining of human endometrial cancer tissue using EGFRvIII antibody (dilution at 1:200)

Western blot analysis of human breast cancer 3 (lane 1), breast cancer 2 (lane 2), breast cancer 1 (lane 3) using EGFRvIII antibody (Dilution at 2 ug/ml)

Immunohistochemical staining of human mammary fibroma tissue using EGFRvIII antibody (dilution of primary antibody - 1:200)

IHC-P image of human mammary fibroma tissue using EGFRvIII antibody (dilution of primary antibody at 1:200)

IHC-P from different cancer patients(Adenocarcinoma).

IHC-P from different cancer patients(Adenocarcinoma).

IHC-P from different cancer patients(Adenocarcinoma).
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Protocol Information
EGFRvIII antibody (orb191506)
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