Engrailed 1 Rabbit Monoclonal Antibody

SKU: orb866424

Description

Anti-Engrailed 1 Rabbit Monoclonal Antibody. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cell Biology, Epigenetics & Chromatin, Neuroscience, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 2

Tested Applications	FC, IP, WB
Dilution Range	WB 1:500-2000 IP 1:50 FC 1:100
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone No.	23E80
Immunogen	A synthesized peptide derived from human Engrailed 1
Target	Homeobox protein engrailed-1
Molecular Weight	55 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ATP-binding cassette sub-family F member 1; ATP-binding cassette 50; TNF-alpha-stimulated ABC protein; ABCF1; ABC50

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

WB analysis of Engrailed 1 Rabbit Monoclonal Antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. Lane 2: mouse brain tissue lysates.

Western blot analysis of Engrailed 1 expression in 293T cell lysate.

Quick Database Links

Gene Symbol

Homeobox protein engrailed-1

UniProt

Q05925

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunoprecipitation