Anti-Gamma Delta TCR [B1.1]

SKU: orb2645762

Description

Images & Validation

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Item 1 of 1

Tested Applications	Blocking, FA, FC, IF, IHC
Reactivity	Human, Primate

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	B1.1
Immunogen	Antigen-specific T cell blasts to poly-18, a polypeptide antigen of defined sequence and conformation, were generated from lymph nodes of antigen primed BALB/cCr mice. These blasts were fused with the BW5147 thymoma to obtain anti-poly-18-reactive T cell hybridomas from which the original antibody was generated.
Target	Gamma Delta TCR

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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TCR γδ; γδ TCR; TCR GD; GD TCR; TCRg/d; T-cell surface glycoprotein CD3 delta chain; CD3D; T3D; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 gamma chain; CD3G; T-cell receptor T3 gamma chain; CD3g; T3G; B1

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Immunofluorescence staining of Jurkat cells with anti-Gamma Delta TCR antibody B1.1. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of B1.1 (orb2645762) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645762, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Gamma Delta TCR

UniProt

P04234, P09693

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence