GTPase HRAS Rabbit Polyclonal Antibody

SKU: orb334478

Description

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse
Reactivity	Human, Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminus of human GTPase HRAS, identical to the related mouse and rat sequences.
Target	GTPase HRas
Molecular Weight	21 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; GTPase HRas, N-terminally processed; HRAS; HRAS1

Quality Guarantee

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Anti-GTPase HRAS antibody, Western blotting. All lanes: Anti GTPase HRAS at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: HEPA Whole Cell Lysate at 40 ug. Predicted bind size: 21 KD. Observed bind size: 21 KD.

IHC analysis of GTPase HRAS using anti-GTPase HRAS antibody. GTPase HRAS was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-GTPase HRAS Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

GTPase HRas

UniProt

P01112

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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