Histone H3 (MonoMethyl-R26) Antibody

SKU: orb412156

Description

The Histone H3 (MonoMethyl-R26) Antibody is suitable for IF, IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic Mono-methylated peptide corresponding to residues surrounding R26 of human Histone H3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Tested Applications	IF, IHC, IP, WB
Dilution range	WB: 1:500-2000, IF/ICC: 1:50-200, IP: 1:50-100
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic Mono-methylated peptide corresponding to residues surrounding R26 of human Histone H3 protein. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Disclaimer	For research use only

Alternative Names

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H3FT; Histone H3.1t; H3/t; H3t; H3/g

Quality Guarantee

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Western blot analysis of Histone H3 (MonoMethyl-R26) expression in Hela (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunofluorescent analysis of Histone H3 (MonoMethyl-R26) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

Q16695

NCBI Gene ID

8290

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

Immunofluorescence

Immunoprecipitation