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HIST3H3 Antibody
SKU: orb412104
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Dilution range | WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200, IP: 1:50-100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic Symmetric Di-methylated peptide corresponding to residues surrounding R8 of human Histone H3 protein. The exact sequence is proprietary. |
| Target | HIST3H3 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−anti-H3FT antibody, anti-Histone H3.1t antibody, anti-H3/t antibody, anti-H3t antibody, anti-H3/g antibody
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Western blot analysis of Histone H3 (Symmetric DiMethyl-R8) expression in Hela (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)
Immunofluorescent analysis of Histone H3 (Symmetric DiMethyl-R8) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
HIST3H3 Antibody (orb412104)
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