HLA-DR/DP Antibody (PE)

Expression profiling on peripheral blood subsets using Anti-human HLA-DR/DP PE antibody (clone MEM-136). HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of anti-human HLA-DR/DP PE antibody (clone MEM-136, 20 µl reagent / 100 µl of stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-human HLA-DR/DP PE antibody (clone MEM-136) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Mouse monoclonal anti-human HLA-DR/DP PE antibody (clone MEM-136) was used in amount of 20 µl in 100 µl of blood sample (2 x 10^6 cells).