Hsp75 TRAP1 Rabbit Monoclonal Antibody

SKU: orb548037

Description

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	IP, WB
Dilution range	WB 1:500-2000 IP 1:20
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	IBD-20
Immunogen	A synthesized peptide derived from human Hsp75
Target	Heat shock protein 75 kDa, mitochondrial
Molecular Weight	35 kDa, 39 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin.
Concentration	0.5mg/ml
Disclaimer	For research use only

Alternative Names

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Heat shock protein 75 kDa, mitochondrial; HSP 75; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1; TRAP1; HSP75

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Western blot analysis of Hsp75 expression in K562 cell lysate (orb548037). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAP1 monoclonal antibody (Catalog # orb548037) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for TRAP1.

Quick Database Links

Gene Symbol

Heat shock protein 75 kDa, mitochondrial

UniProt

Q12931

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunoprecipitation

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