Anti-HSPB1 [SAIC-23B-22]

SKU: orb1089992

Description

Rabbit monoclonal antibody to HSPB1

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

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Item 1 of 3

Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone No.	SAIC-23B-22
Immunogen	Peptide "DGVVEITGK" derived from Heat shock protein beta-1, involved in stress resistance and actin organisation, conjugated to KLH.
Target	HSPB1
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Heat shock protein beta-1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSP27; HSP28

Quality Guarantee

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Flow cytometry using the Anti-HSPB1 antibody SAIC-23B-22. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-23B-22 (orb1089992, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-HSPB1 SAIC-23B-22. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-23B-22 (orb1089992) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nucleoli, nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089992, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-HSPB1 antibody SAIC-23B-22. Human heart(A) (0.1 µg/ml) and human placenta(B) (0.3 ug/ml) tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-23B-22 (orb1089992), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792

UniProt Details

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No UniProt data available

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