CD123 Antibody (PE)

SKU: orb154527

Description

Mouse monoclonal antibody conjugated to PE which recognizes CD123 that is also known as IL-3 receptor subunit alpha, IL-3R-alpha and IL3RA, is a single-pass type I membrane protein which is a member of the type I cytokine receptor family and Type 5 subfamily. CD123 is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. CD123 is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes.

Research Area

Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	FC
Reactivity	Human
Application Notes	Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	6H6
Immunogen	IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
Target	CD123
Purification	Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	PE

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Disclaimer	For research use only

Alternative Names

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IL3RA, MGC34174, hIL-3Ra

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Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD123 positive Basophil granulocytes(red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD123 (6H6) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Quick Database Links

Gene Symbol

CD123

UniProt

P26951

NCBI Gene ID

3563

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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