CD135 Antibody (FITC)

SKU: orb154474

Description

Mouse monoclonal antibody conjugated to FITC which recognizes CD135 that belongs to the protein kinase superfamily, Tyr protein kinase family. It expressed in bone marrow cells. Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. CD135 plays a role in cell survival, proliferation, and differentiation. CD135 is also important for lymphocyte (B cell and T cell) development.

Research Area

Cancer, Epigenetics, Infectious Diseases

Images & Validation

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Tested Applications	FC
Reactivity	Human
Application Notes	Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	BV10A4
Immunogen	BV-173 leukemic cell line
Target	CD135
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Disclaimer	For research use only

Alternative Names

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FLT3, FLK2, STK1

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Separation of REH cells stained using anti-human CD135 (BV10A4) FITC antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled) from REH cells unstained by primary antibody (black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of REH cellular suspension stained using anti-human CD135 (BV10A4) FITC antibody (4 μl reagent per million cells in 100 μl of cell suspension).

Quick Database Links

Gene Symbol

CD135

UniProt

P36888

NCBI Gene ID

2322

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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