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CD28 Antibody
SKU: orb43723
Description
Research Area
Epigenetics, Stem Cells
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, IHC-Fr, IP, WB |
|---|---|
| Reactivity | Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Clone No. | CD28.2 |
| Immunogen | DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA |
| Target | CD28 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−TP44
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Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1.6 µg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD28 (CD28.2) purified antibody (concentration in sample 1.6 µg/ml) GAM APC.
Western blotting analysis of human CD28 using mouse monoclonal antibody CD28.2 on lysates of Jurkat cells and THP1 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD28 was detected around 35-40 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
CD28 Antibody (orb43723)
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