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Goat anti-CD80 Antibody
SKU: orb20356
Description
Research Area
Epigenetics, Infectious Diseases, Stem Cells
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, FC, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:4000, WB: 0.3-1 μg/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | CD80 |
| Protein Sequence | HLRVNQTFNWNTTK |
| Molecular Weight | 33 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti CD80 antibody, anti CD80 molecule antibody, anti CD28LG antibody, anti CD28LG1 antibody, anti LAB7 antibody, anti B-lymphocyte activation antigen B7 antibody, anti CD80 antigen antibody, anti CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody, anti costimulatory factor CD80 antibody, anti costimulatory molecule variant IgV-CD80 antibody
Quality Guarantee
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0.5 µg/ml staining of K562 (A) and U937 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
3 ug/ml staining of Human Lymph Node (A), Bone Marrow (B) and 1.5 ug/ml Spleen (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
5 µg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Goat anti-CD80 Antibody (orb20356)
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