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CD3 Antibody
SKU: orb44559
Description
Research Area
Epigenetics, Immunology, Stem Cells
Images & Validation
−Item 1 of 3
| Tested Applications | FA, FC, IHC-Fr, IP |
|---|---|
| Reactivity | Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Clone No. | UCHT1 |
| Immunogen | human thymocytes followed by Sezary T cells |
| Target | CD3 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD3E, T3E, TCRE
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Mass cytometry (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD3 (UCHT1) Yb174. Gated on singlets.
Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (UCHT1) purified antibody (low endotoxin, concentration in sample 2 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (UCHT1) purified antibody (low endotoxin, concentration in sample 2 μg/ml) GAM APC.
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Datasheet
Product Information
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Protocol Information
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IP
Immunoprecipitation
CD3 Antibody (orb44559)
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