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CD314 Antibody

SKU: orb154374

Description

Mouse monoclonal antibody to CD314 which is also known as BAT221, NKG2D a cell surface receptor of the type II transmembrane receptor NKG2 family. CD314 is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion.

Research Area

Epigenetics, Immunology

Images & Validation

Tested ApplicationsFA, FC, IHC-Fr, IP
ReactivityHuman
Application Notes
Functional application: Blocking of ligand binding. Immunohistochemistry: Recommended dilution: 5-10 μg/ml. Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1 kappa
Clone No.1D11
ImmunogenNKL cell line
TargetCD314
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

NKG2D, KLRK1, KLR

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CD314 Antibody

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC and anti-human CD56 (LT56) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

CD314 Antibody

Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.

CD314 Antibody

Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD314 (1D11) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
View Protocol
IP
Immunoprecipitation
View Protocol

CD314 Antibody (orb154374)

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0.1 mg
¥ 3,640.00
DispatchUsually dispatched within 5-10 working days weeks.
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