SIGLEC10 Antibody (PE)

Expression profiling on peripheral blood subsets using Anti-SIGLEC10 PE antibody (clone 5G6). HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of anti-human SIGLEC10 PE antibody (clone 5G6, 10 μl reagent / 100 μl of stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Anti-SIGLEC10 PE antibody (clone 5G6) works in flow cytometry application. Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Mouse monoclonal anti-human SIGLEC10 PE antibody (clone 5G6) was used in amount of 10 µl in 100 µl of blood sample (2 x 10^6 cells).