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Anti-Human Ephrin Type A receptor 2 [1C1]
SKU: orb348860
Featured
Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Signal Transduction, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Reactivity | Human, Mouse |
Key Properties
−| Host | Human |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 1C1 |
| Immunogen | 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1. |
| Target | Human Ephrin Type A receptor 2 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EphA2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
Similar Products
−- Item 1 of 2
Anti-Human Ephrin Type A receptor 2 [1C1] [orb348887]
ELISA, FC, IF, WB
Human, Mouse
Human
Monoclonal
Unconjugated
0.2 mg
![Anti-Human Ephrin Type A receptor 2 [1C1]](/images/pub/media/catalog/product/NewWebsite/35/orb348887_1.png)
![Anti-Human Ephrin Type A receptor 2 [1C1]](/images/pub/media/catalog/product/NewWebsite/35/orb348887_2.png)
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![Anti-Human Ephrin Type A receptor 2 [1C1]](/images/pub/media/catalog/product/NewWebsite/35/orb348860_1.png)
Immunofluorescence staining of fixed LL/2 cells with anti-Human Ephrin Type A receptor 2 antibody 1C1 Fixed murine Lewis Lung cells were stained with the chimeric mouse IgG1 version of 1C1 at 2 µg/ml and a donkey anti-mouse IgG-AF488 (20 µg/ml) for 90 mins. Nuclei stained with Hoechst. Cells captured at 20x magnification. Panel A shows a merge of the fluorescence channels, whilst panel B shows the DIC image. In this fixed sample the cell membrane is clearly outlined.
![Anti-Human Ephrin Type A receptor 2 [1C1]](/images/pub/media/catalog/product/NewWebsite/35/orb348860_2.png)
Immunofluorescence staining of live LL/2 cells with anti-Human Ephrin Type A receptor 2 antibody 1C1 Live murine Lewis Lung cells were stained with the chimeric mouse IgG1 version of 1C1 at 2 µg/ml and a donkey anti-mouse IgG-AF488 (20 µg/ml) for 90 mins. Nuclei stained with Hoechst. Cells captured at 20x magnification. Panel A shows the AF488 signal, panel B the DIC image and panel C a merge of fluorescence (AF488 + Hoechst) with the DIC image. In this live sample, antibody internalisation is observed.
Quick Database Links
Gene Symbol
Human Ephrin Type A receptor 2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Peng, Li et al. Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody J Mol Biol, 413, 390-405 (2011)
Anti-Human Ephrin Type A receptor 2 [1C1] (orb348860)
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