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Anti-IL-10 [9D7]
SKU: orb1089968
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IF, IHC, IP |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Rat |
|---|---|
| Clonality | Monoclonal |
| Isotype | Rat IgG |
| Clone No. | 9D7 |
| Immunogen | Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine. |
| Target | IL-10 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Human cytokine synthesis inhibitory factor;GVHDS; IL10; Interleukin 10; Interleukin-10; IL10A; TGIF; JES3-9D7; JES3 9D7;
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ELISA of anti-IL-10 antibody on human rhIL-10. Binding curves of the human IgG1 chimeric version of the anti-IL-10 antibody 9D7 (blue line) and isotype control (orb256383, anti-fluorescein; orange line) to an ELISA plate coated with human rhIL-10 at a concentration of 2.5 µg/ml. A 3-fold serial dilution from 3000 ng/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an anti-human IgG/HRP antibody was used.
Quick Database Links
Gene Symbol
IL-10
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Anti-IL-10 [9D7] (orb1089968)
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