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Anti-IL-10R [1B1.3a]

SKU: orb758898

Description

Rabbit monoclonal antibody to IL-10R

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

Tested ApplicationsBlocking, FC
ReactivityMouse

Key Properties

HostRat
ClonalityMonoclonal
IsotypeRat IgG
Clone No.1B1.3a
ImmunogenThis antibody was raised by immunising rats with the purified recombinant extracellular region of mouse Interleukin 10 Receptor.
TargetIL-10R
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

CD210; Interleukin-10 receptor subunit; Interleukin-10 receptor subunit alpha; Il10ra Il10r; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; IBI-3A; 1B1-3A

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    Blocking,  FC

    Mouse

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    Monoclonal

    Unconjugated

    0.2 mg
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Anti-IL-10R [1B1.3a]

Flow cytometry using the Anti-IL-10R antibody 1B1.3a. RAW264.7 cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 1B1.3a (orb758898, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Anti-IL-10R [1B1.3a]

Immunofluorescence staining of fixed RAW264.7 cells with anti-IL-10R antibody 1B1.3a. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 1B1.3a (orb758898) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758898, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

UniProt Details

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Protocol Information

FC
Flow Cytometry
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Anti-IL-10R [1B1.3a] (orb758898)

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Available Sizes

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0.2 mg
¥ 7,800.00
DispatchUsually dispatched within 5-10 working days
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