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IL-12RB2 Rabbit Polyclonal Antibody
SKU: orb1880934
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB (1/500 - 1/1000), FC (1/10 - 1/30) |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human IL-12RB2. The exact sequence is proprietary. |
| Target | IL12RB2 |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-12 receptor subunit beta-2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2
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Western blot analysis of IL-12RB2 expression in THP1 (A), A431 (B), Jurkat (C), MOLT4 (D) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 95 kD)
Flow cytometric analysis of A431 cells using Anti-IL-12RB2 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IL-12RB2 Rabbit Polyclonal Antibody (orb1880934)
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