Anti-IL-17F [8F5.1A9]

SKU: orb758851

Description

Mouse monoclonal antibody to IL-17F

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	FC
Reactivity	Mouse

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	8F5.1A9
Immunogen	This antibody was raised by immunising mice with murine IL-17F coupled to ovalbumin to generate a highly immunogenic complex.
Target	IL-17F
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Interleukin-17F; IL17F

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Flow-cytometry using the Anti-IL-17F antibody 8F5.1A9. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 8F5.1A9 (orb758852, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

IL-17F

UniProt

Q7TNI7

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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