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Anti-IL-18 [125-2H]

SKU: orb613779

Description

Mouse monoclonal antibody to Interferon gamma

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, IF, IP
ReactivityHuman

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.125-2H
ImmunogenFusion of splenocytes from mice immunized with human IL-18, with SP2/O mouse myeloma cells.
TargetIL-18
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Iboctadekin; Interleukin 18 (Interferon-gamma-inducing factor); IGIF; IL1F4; IL-1g

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    Mouse

    Monoclonal

    Unconjugated

    0.2 mg
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Anti-IL-18 [125-2H]

Western Blot using anti-IL18 antibody 125-2H HeLa cell lysate samples (35 µg protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H (orb613780) at 2 µg/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. orb613780 successfully detected IL18 in HeLa cell lysate samples.

UniProt Details

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Protocol Information

IF
Immunofluorescence
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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IP
Immunoprecipitation
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Anti-IL-18 [125-2H] (orb613779)

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Available Sizes

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0.2 mg
¥ 7,800.00
Free Secondary Antibody (20 ul)0/0

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