Anti-IL-6R [D7715A7]

SKU: orb758841

Description

Rabbit monoclonal antibody to IL-6R

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	Blocking, FC
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG
Clone No.	D7715A7
Immunogen	This antibody was raised by immunising Lewis rats with OKT4 cells.
Target	IL-6R
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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CD126; 15A7; IL-6Ra; IL6R; Interleukin-6 receptor subunit alpha; Il6ra; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1

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Flow-cytometry using the Anti-IL-6R antibody D7715A7. Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (orb758841, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (orb758841) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758841, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

IL-6R

UniProt

P22272

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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