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Anti-IL-8RB [HC2]

SKU: orb758963

Description

Rabbit monoclonal antibody to IL-8RB

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsFC
ReactivityHuman

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeMouse IgG
Clone No.HC2
ImmunogenThis antibody was raised by immunizing mice with human IL-8 (interleukin-8) receptor, type B.
TargetIL-8RB
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

CXCR-2; CD182; C-X-C chemokine receptor type 2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; IL-8 receptor type 2

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Anti-IL-8RB [HC2]

Flow cytometry using the Anti-IL-8RB antibody HC2. Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of HC2 (orb758963, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Anti-IL-8RB [HC2]

Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RB antibody HC2. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of HC2 (orb758963) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758963, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

UniProt Details

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Protocol Information

FC
Flow Cytometry
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Anti-IL-8RB [HC2] (orb758963)

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Available Sizes

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0.2 mg
¥ 7,800.00
DispatchUsually dispatched within 5-10 working days
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