Lymphotactin/XCL1 Antibody

Sandwich ELISA - Recombinant human Lymphotactin/XCL1 protein standard curve. Use in combination with reagents from Human Lymphotactin/XCL1 ELISA Kit EZ-Set (DIY Antibody Pairs).

Western blot analysis of Lymphotactin using anti-Lymphotactin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lymphotactin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lymphotactin at approximately 13 kDa. The expected band size for Lymphotactin is at 13 kDa.