Mesothelin Antibody

SKU: orb757396

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Description

Anti-MSLN antibody(DM73), Rabbit mAb

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, FC, WB
Dilution range	ELISA 1/5000-10000\|Flow Cyt 1/100
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	DM73
Target	Mesothelin
Purification	Purified from cell culture supernatant by affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Disclaimer	For research use only

Alternative Names

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MSLN, Mesothelin, MPF

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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human MSLN protein, mFc-His tagged protein orb689392 can bind Rabbit anti-MSLN monoclonal antibody (clone: DM73) in a linear range of 1-100 ng/ml.

Expi 293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1 μg/ml (clone: DM73) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody (clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of rabbit anti-human Mesothelin mAb (orb757396). (A) orb757396 does not bind to 293T cells that do not express Mesothelin. (B) A clear peak shift of orb757396 was seen compared to the control when incubated with Mesothelin-expressing Hela cells, indicating strong binding of orb757396 to Mesothelin. Antibodies were incubated at 2 μg/mL.

Anti-MSLN antibody (SKU# orb757396) at 1/1000 dilution.Lane: HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate.Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution.Predicted band size: 69 kDa.Observed band size: 50 kDa

Quick Database Links

Gene Symbol

Mesothelin

UniProt

Q13421

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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