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CD3 Antibody

SKU: orb44593

Description

Hamster monoclonal antibody to CD3

Research Area

Epigenetics, Immunology, Stem Cells

Images & Validation

Tested ApplicationsFC, ICC, IHC-Fr, IP
ReactivityMouse
Application Notes
Flow cytometry: Recommended dilution: 1-2 μg / ml (million cells). Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg protein in 1 ml of a cell lysate.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeArmenian Hamster IgG kappa
Clone No.145-2C11
ImmunogenMouse BM10-37 cytotoxic T lymphocytes
TargetCD3
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD3E, T3E, TCRE

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CD3 Antibody

Separation of murine CD3 positive splenocytes (red-filled) from CD3 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) purified antibody (concentration in sample 4 μg/ml) DAR APC.

CD3 Antibody

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD3 (145-2C11) purified antibody (concentration in sample 4 μg/ml) DAR APC.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol
IP
Immunoprecipitation
View Protocol

CD3 Antibody (orb44593)

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Available Sizes

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0.1 mg
¥ 2,470.00
DispatchUsually dispatched within 5-10 working days.
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