p62 Rabbit Monoclonal Antibody

SKU: orb1474424

Description

The p62 Antibody is suitable for IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein of human SQSTM1/p62 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Tested Applications	IF, IP, WB
Dilution Range	WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Immunogen	Recombinant protein of human SQSTM1/p62
Target	SQSTM1
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ORCA; OSIL; Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62

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Western blot analysis of p62 expression in A549 (A), HL60 (B), U2OS (C) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 62 kD)

Immunofluorescent analysis of p62 staining in U87MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

SQSTM1

UniProt

Q13501

NCBI Gene ID

8878

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

Immunofluorescence

Immunoprecipitation