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PCNA Antibody
SKU: orb402808
Description
Research Area
Cancer, DNA Damage, Epigenetics, Infectious Diseases, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Reactivity | Drosophila, Gallus, Human, Mouse, Primate, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a kappa |
| Clone No. | PC10 |
| Immunogen | recombinant rat PCNA |
| Target | PCNA |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ATLD2
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Separation of K562 cells stained using anti-PCNA (PC10) purified antibody (concentration in sample 4 µg/ml, GAM FITC, red-filled) from K562 cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).
Immunocytochemistry staining of PCNA in PHA-stimulated lymphocytes using mouse monoclonal antibody PC10 (diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200); DNA stained with propidium iodide. Colocalization of green PCNA signal and red DNA signal indicated as yellow.
Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-PCNA (PC10), 10 µg/ml.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
PCNA Antibody (orb402808)
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