You have no items in your shopping cart.
Phospho-Hsp27 (S82) HSPB1 Rabbit Monoclonal Antibody
SKU: orb548613
Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB 1:500-2000 IP 1:50 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | FGD-8 |
| Immunogen | A synthesized peptide derived from human Phospho-Hsp27 (S82) |
| Target | Heat shock protein beta-1 |
| Molecular Weight | 185 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin. |
| Concentration | 0.5mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28
Similar Products
−
Phospho-Hsp27 (S82) Rabbit monoclonal antibody [orb2298178]
IP, WB
Human, Mouse, Rat
Rabbit
Monoclonal
Unconjugated
25 μl, 50 μl, 100 μlPhospho-HSP27 (Ser82) Recombinant Rabbit Monoclonal Antibody [orb559186]
FC, WB
Rat
Human, Mouse
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western blot analysis of Phospho-Hsp27 (S82) expression in HeLa cell lysate treated with heat shock (orb548613). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # orb548613) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for HSPB1.
Quick Database Links
Gene Symbol
Heat shock protein beta-1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Phospho-Hsp27 (S82) HSPB1 Rabbit Monoclonal Antibody (orb548613)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 2-4 weeks