Anti-PRDM9 [RAB-C370]

SKU: orb1089927

Description

Human polyclonal antibody to PRDM9

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 3

Tested Applications	ChIP, ELISA, IF
Reactivity	Human

Key Properties

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Host	Human
Clonality	Monoclonal
Isotype	Fab fragment
Clone No.	RAB-C370
Immunogen	This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM9 protein under non-denaturing conditions.
Target	PRDM9
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Histone-lysine N-methyltransferase PRDM9; PR domain-containing protein 9; PR domain zinc finger protein 9; Protein-lysine N-methyltransferase PRDM9; [histone H3]-lysine36 N-trimethyltransferase PRDM9; [histone H3]-lysine4 N-trimethyltransferase PRDM9; [histone H3]-lysine9 N-trimethyltransferase PRDM9; [histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9; [histone H4]-lysine20 N-methyltransferase PRDM9; EC:2.1.1.-; anti-PRDM9-RAB-C370; PRDM9-A003-1459

Quality Guarantee

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Flow cytometry using the Anti-PRDM9 antibody RAB-C370. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of RAB-C370 (orb1089950, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HEK293 cells with anti-PRDM9 RAB-C370. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C370 (orb1089950) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089950, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Immunofluorescence staining of HeLa cells with anti-PRDM9 RAB-C370. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C370 (orb1089950) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089950, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

PRDM9

UniProt

Q9NQV7

UniProt Details

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No UniProt data available

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Protocol Information

Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ChIP

Chromatin Immunoprecipitation

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